Backup is what someone gives when they help or support you. A brand new doctor might need some backup in the emergency room for the first few weeks.
Use the noun backup when you talk about extra assistance or help. Police officers call for backup when they're not sure they can handle a situation on their own, and store cashiers need backup when things get busy and customers start lining up. Another kind of backup is the one you do on your computer — in this case, a backup is the process of copying your data in case you lose the original files.